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65906

Sigma-Aldrich

鬼笔环肽–Atto 647N

BioReagent, suitable for fluorescence, ≥80% (HPLC)

同義詞:

Atto 647N, Atto 647N-鬼笔环肽

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About This Item

分類程式碼代碼:
12171501
NACRES:
NA.32

產品線

BioReagent

化驗

≥80% (HPLC)

製造商/商標名

ATTO-TEC GmbH

&lambda ;

in methanol

紫外吸收

λ: 640-646 nm Amax

適合性

suitable for fluorescence

檢測方法

fluorometric

儲存溫度

−20°C

一般說明

Phalloidin–Atto 647N 是一种新的荧光标记物,靶向红色光谱区域。Atto 647N 是一种阳离子染料,在耦合后,它携带 +1 的净电荷。与大多数 Atto 标签一样,Atto 647N 的吸收和荧光与 2-11 之间的 pH 无关。Atto 647N 是以含有两种具有相同荧光和吸收特性的异构体的混合物的形式提供的。Atto 标记物具有刚性结构,不显示任何顺反异构化。

應用

Phalloidin–Atto 647Nis,设计用于标记 DNA、RNA 或蛋白质。phaloidin 的荧光结合物用于标记肌动蛋白丝,用于组织学应用。鬼笔环肽的某些结构特征是与肌动蛋白结合所必需的。

特點和優勢

Atto 488-鬼笔环肽的特点:
  1. 强吸光度:
  2. 高荧光量子产率。
  3. 高光稳定性。
  4. 最低三重态。
  5. 好溶解性。
  6. 出色的抗臭氧性能。

法律資訊

本品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


分析證明 (COA)

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BMC biology, 6, 40-40 (2008-09-17)
Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry
Benedetta Artegiani et al.
Nature cell biology, 22(3), 321-331 (2020-03-04)
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation
Francesca Farina et al.
The EMBO journal, 38(11) (2019-04-25)
Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule
Laura F Corns et al.
Nature communications, 9(1), 4015-4015 (2018-10-03)
In the adult auditory organ, mechanoelectrical transducer (MET) channels are essential for transducing acoustic stimuli into electrical signals. In the absence of incoming sound, a fraction of the MET channels on top of the sensory hair cells are open, resulting

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