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Key Documents

52403

Sigma-Aldrich

Anti-Mouse IgG−Abberior® STAR 580 antibody produced in goat

for STED application

同義詞:

Abberior® STAR 580-Anti-Mouse-IgG antibody produced in goat

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

goat

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse

存貨情形

available only in USA and Canada

濃度

~1 mg/mL

螢光

λex 587 nm; λem 607 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3, Atto 590 or Alexa Fluor 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 587 (PBS pH 7.4), 584 (H2O), 584 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 85 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,17 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 607 (PBS pH 7.4), 607 (H2O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)

應用

Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections.

適合性

Designed and tested for fluorescent super-resolution microscopy

分析報告

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法律資訊

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy3 is a trademark of Cytiva
abberior is a registered trademark of Abberior GmbH

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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