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Merck
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文件

50980

Sigma-Aldrich

异硫氰酸胍

BioUltra, for molecular biology, ≥99.0% (AT)

同義詞:

硫氰酸亚脲, 硫氰酸胍

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About This Item

線性公式:
NH2C(=NH)NH2 · HSCN
CAS號碼:
分子量::
118.16
Beilstein:
3563461
EC號碼:
MDL號碼:
分類程式碼代碼:
12352107
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

產品線

BioUltra

化驗

≥99.0% (AT)

形狀

solid

雜質

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

燃燒殘留物

≤0.5% (as SO4)

損耗

≤0.1% loss on drying, 20 °C (HV)

pH值

4.5-7.0 (25 °C, 6 M in H2O)

mp

117-120 °C
120-122 °C (lit.)

溶解度

H2O: 6 M at 20 °C, clear, colorless

負離子痕跡

chloride (Cl-): ≤5000 mg/kg
sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

吸收

cut-off at 280 nm in H2O at 6 M

SMILES 字串

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI 密鑰

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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應用

离液剂和强效变性剂;溶解细胞。

其他說明

RNA 的分离 ;从富含核糖核酸酶和蛋白聚糖的组织中提取全长 RNA 模板进行逆转录

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

安全危害

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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J C Groppe et al.
Analytical biochemistry, 210(2), 337-343 (1993-05-01)
RNA isolated by conventional guanidinium isothiocyanate methods from tissues of a mollusc (red abalone: Haliotis rufescens) is largely degraded and discolored by contaminants. These contaminants are associated with inhibition of reverse transcriptase, prevent accurate spectrophotometric determination of RNA concentration, and
Turpen, T.H. and Griffith, O.M.
Biotechniques, 4, 11-11 (1986)
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Yuji Suzuki et al.
Bioscience, biotechnology, and biochemistry, 72(7), 1951-1953 (2008-07-08)
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds

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