儲存溫度
2-8°C
一般說明
1 ml settled resin of FabRICATOR (IdeS) immobilized on highly cross-linked agarose and 1 ml CapureSelect IgG-Fc affinity ligand immobilized on agarose. Agarose resins are suspended in 20% ethanol/water (V/V)
應用
FragIT™ MidiSpin Kit (cleaving and purifying up to 10mg IgG) can be used for antibody characterization.
生化／生理作用
IgG fragmentation is accompanying with adverse consequences like low yield, time consuming optimization and operation at low pH with loss of immunoreactivity. These consequences can be overcome with FragIT™ MidiSpin Kit, which comprises of pre-filled Fabricator enzyme covalently coupled to agarose beads that facilitates the cleavage of IgG (cleaving and purifying up to 10mg IgG) to generate pure F(ab′)2 and Fc fragments. FabRICATOR is a digestive enzyme that digests IgG specifically below the hinge region so there is no risk of over digestion if incubation time exceeds.
單位定義
1 ml of settled resin fragments ≥ 95% of 5 mg of human IgG in 15 minutes at 25°C, pH 6.6 as monitored by SDS-PAGE.
分析報告
Enzymatic activity: corresponds to requirements
法律資訊
FragIT is a trademark of Genovis AB
訊號詞
Danger
危險聲明
危險分類
Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
57.2 °F - closed cup
閃點（°C）
14.0 °C - closed cup
Lukmanee Tradtrantip et al.
Molecular pharmacology, 83(6), 1268-1275 (2013-04-11)
Neuromyelitis optica (NMO) is an inflammatory demyelinating disease of the central nervous system caused by binding of pathogenic IgG autoantibodies (NMO-IgG) to astrocyte water channel aquaporin-4 (AQP4). Astrocyte damage and downstream inflammation require NMO-IgG effector function to initiate complement-dependent cytotoxicity
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