推薦產品
重組細胞
expressed in E. coli
形狀
crystals
powder
比活性
≥2.0 U/mg
分子量
41 kDa
儲存溫度
2-8°C
一般說明
腈水解酶属于C-N水解酶超家族。它具有催化三联体基序：谷氨酸，赖氨酸和半胱氨酸，分布在真菌，植物和动物中。该蛋白的等电点（pI）为8.1。天然聚丙烯酰胺凝胶电泳（PAGE）。
應用
腈水解酶可用于丙烯酰基交联纤维素二醛（ACCD）的固定化，用于催化活性研究。
生化／生理作用
腈水解酶催化氨的释放和腈形成羧酸。腈水解酶的寡聚结构组织对其功能至关重要。它显示出广泛的底物特异性。
包裝
无底玻璃瓶。内含物装在插入的融合锥内。
單位定義
1U对应于在pH 7.2和25℃下每分钟释放1 μmol氨且丙烯腈转化为丙烯酸的酶的量
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Zhi-Qiang Liu et al.
PloS one, 8(6), e67197-e67197 (2013-07-05)
In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase
Zhi-Jun Zhang et al.
Journal of biotechnology, 152(1-2), 24-29 (2011-01-26)
The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high concentrations (>300mM), which resulted in a low yield of the target product (R)-(-)-mandelic acid. To relieve
Y V D Nageshwar et al.
Bioprocess and biosystems engineering, 34(5), 515-523 (2010-12-29)
Microbial nitrilases are biocatalysts of interest and the enzyme produced using various inducers exhibits altered substrate specificity, which is of great interest in bioprocess development. The aim of the present study is to investigate the nitrilase-producing Alcaligenes faecalis MTCC 10757
Chunsheng Yang et al.
Applied biochemistry and biotechnology, 165(7-8), 1556-1567 (2011-09-23)
The characteristics of the new nitrilase-producing strain Rhodobacter sphaeroides LHS-305 were investigated. By investigating several parameters influencing nitrilase production, the specific cell activity was ultimately increased from 24.5 to 75.0 μmol g(-1) min(-1), and hereinto, the choice of inducer proved
Pablo Soriano-Maldonado et al.
Applied and environmental microbiology, 77(16), 5761-5769 (2011-06-28)
Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate
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