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Key Documents

00289

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 512 antibody produced in goat

for STED application

同義詞:

Abberior® STAR 512-Anti-Rabbit IgG antibody produced in goat

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

goat

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rabbit

濃度

~1 mg/mL

螢光

λex 511 nm; λem 530 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto 514 or Alexa Fluor 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

分析報告

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法律資訊

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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S. M. Polyakova, et al.,
European Journal of Organic Chemistry, 30, 5162-5177 (2009)
Gyuzel Yu Mitronova et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to

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