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S9430

Sigma-Aldrich

SYBR®Green I核酸凝胶染液

greener alternative

10,000 × in DMSO

同義詞:

DNA stain, SYBR® green gel dye, safer gel stain

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About This Item

CAS號碼:
163795-75-3
MDL號碼:
MFCD00284715
分類程式碼代碼:
12171500
NACRES:
NA.52
暫時無法取得訂價和供貨情況

形狀

solution

品質等級

200

用途

1.0 mL sufficient for 100 mini-gels

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

濃度

10,000 × in DMSO

技術

PCR: suitable

環保替代類別

Aligned,

儲存溫度

−20°C

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相關類別

一般說明

SYBR® Green I是一种特制的不对称花菁染料，用于检测核酸。这种成分由N-烷基化苯并噻唑或苯并噁唑环系统组成，通过单次甲基桥连接吡啶鎓或喹啉鎓环系统。[1]SYBR Green I与dsDNA的小沟结合，激发波长480 nm。其荧光发射峰波长为520nm。[2]

應用

SYBR® Green I核酸凝胶染色剂已用于：
  • dsDNA定量[3]
  • 聚合酶链式反应（PCR），用于DNA染色[4]
  • Comet测定技术[5]
  • 评估精子膜的完整性[6]
  • 扩增DNA 目视检查（环介导等温扩增（LAMP）法）[7]
  • 用作流式细胞术中的荧光染料[8]
  • 实时定量逆转录聚合酶链式反应（qRT-PCR），用于逆转录cDNA染色[8]

特點和優勢

  • 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
  • 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA，无需任何预洗涤步骤
  • 在 Ames 试验中，它的致突变性比溴化乙锭小
  • 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
  • 适用于 DNA 数量有限的许多应用
  • SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性，包括 Hind III 和 EcoR I
  • 这种染色剂无需通过凝胶酶解和连接技术去除
  • SYBR Green I 是溴化乙锭染色的绿色替代产品

儲存和穩定性

将产品储存在–20 °C。稀释后的染色溶液避光可在2–8 °C保存数周或室温保存3-4天。

法律資訊

SYBR is a registered trademark of Life Technologies

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

201.2 °F - closed cup

閃點（°C）

94 °C - closed cup

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000400498
0000400498
0000371131
SLCP5377
MKCW7943

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Deborah Traversi et al.
Scientific reports, 10(1), 17566-17566 (2020-10-18)
Type 1 diabetes (T1D) is a common autoimmune disease that is characterized by insufficient insulin production. The onset of T1D is the result of gene-environment interactions. Sociodemographic and behavioural factors may contribute to T1D, and the gut microbiota is proposed
Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
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Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

SeqPlex 增强型DNA扩增试剂盒 (SEQXE)实验方案

面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

完整全转录组扩增试剂盒实验方案(WTA2)

WTA2（一种全转录组扩增（WTA）方法）能够在区区4小时内，代表性地扩增出纳克级的总RNA而没有3'偏差。

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相關內容

Hot Start dNTP protocol to reduce non-specific amplification

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Questions

1–4 of 4 Questions  

  1. Does Millipore Sigma offer SAFE Red loading dye?

    1 answer

    1. SAFE Red loading dye is not offered by Millipore Sigma.

      Helpful?

  2. What is the molarity of this product?

    1 answer

    1. This product is a 10,000X concentrated solution suitable to prepare 10L of staining solution for in-gel staining of DNA and RNA. The specific concentration is considered proprietary. Published working concentrations for solutions of Sybr Green I range from 0.5 - 1.0 ug/ml. The molecular weight as published by Chebi, is 509.74 g/mol. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/282/803/s9430pis-ms.pdf

      Please see the link below to review the Chebi profile:
      https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461


      chebi file
      https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:51461 MW 509.74
      Science direct
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/sybr-green-i
      'working' concentration 0.5 - 1.0 ug/ml

      Helpful?

  3. Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?

    1 answer

    1. Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine.  A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine.  This will allow for better staining.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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