67438

C. perfringens Millichrome^™ plus Agar Base

selective for bacteria: Clostridium perfringens, suitable for microbiology

• 同義詞: C. perfringens Chromogenic Agar Base, C. perfringens Differential Agar Base, C. perfringens Selective Agar Base
• 分類程式碼代碼: 41161501
• NACRES: NA.74

屬性

• agency: EP; JP
• 品質等級: 200
• 滅菌相容性: autoclavable compatible
• 形狀: dry powder
• 儲存期限: limited shelf life, expiry date on the label
• 成份: Agar, 15.0 g/L ; Chromogenic and Selective mix, 1.4 g/L ; Peptones and yeast; extract, 25.0 g/L ; Salts, 6.0 g/L
• 製造商／商標名: MilliChrome^™
• 技術: microbiological culture: suitable
• 最終pH值: 7.6±0.2 (25 °C)
• 溶解度: deionized water: 50.9 g/L
• 應用: food and beverages; pharmaceutical; water monitoring; microbiology
• 運輸包裝: ambient
• 儲存溫度: 15-25°C
• 適合性: selective for bacteria: Clostridium perfringens

描述

一般說明

C. perfringens Millichrome^™ plus Agar Base is a selective chromogenic medium used for the detection and enumeration of Clostridium perfringens. The medium contains peptones and yeast extract that provide nitrogenous nutrients for growth, sodium chloride for osmotic balance, chromogenic substrates for color differentiation, and selective agents to inhibit the growth of other organisms. The medium also contains some special growth factors for the growth of C. perfringens, and agar as the solidifying agent.

應用

C. perfringens Millichrome^™ plus Agar Base is suitable for the detection and enumeration of Clostridium perfringens.

準備報告

Step 1 (Preparation of the base C. perfringens Millichrome^™ plus Agar Base)

• Disperse slowly 50.9 g of powder base in 1 L of purified water.
• Stir until agar is well thickened.
• Heat and bring to boiling (100 °C) while swirling or stirring regularly.
• AUTOCLAVE at 121 °C during 15 min.
• Mix when removing it from the autoclave.
• Cool in a water bath to 45/50 °C, swirling or stirring gently.

Step 2 (Preparation of the Supplement 1)

• In a transparent vessel, add 2 g of C. perfringens Millichrome^™ plus Agar Base supplement 1 in 20 mL of purified water.
• Swirl well until complete dissolution.
• Filter to sterilize at 0.45 μm.

Step 3 (Preparation of the Supplement 2)

• Add 0.12 g of C. perfringens Millichrome^™ plus Agar Base supplement 2 in 1 mL of purified water.
• Filter to sterilize at 0.45 μm

Step 4 (Mixing of the prepared base and the supplements 1 and 2)

• Add 20 mL/L of the supplement 1 solution to the melted base at 45/50 °C.
• Add 1 mL/L of the supplement 2 solution to the melted base at 45/50 °C.
• Swirl or stir gently to homogenize.
• Pour into sterile Petri dishes
• Let it solidify and dry.

儲存和穩定性

• Store prepared media below 8°C, protected from direct light (max. 1 month).
• Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.

分析報告

The medium is specific for C. perfringens and can differentiate it from other organisms based on the orange color of the colonies.

法律資訊

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3