66759

Lactose sulfite medium

suitable for microbiology

• 同義詞: LS
• 分類程式碼代碼: 41171607
• NACRES: NA.21

屬性

• 儲存期限: limited shelf life, expiry date on the label
• 成份: Cysteine hydrochloride, 0.3 g/L ; Lactose monohydrate, 10 g/L ; Pancreatic digest of casein, 5 g/L ; Sodium chloride, 2.5 g/L ; Yeast extract, 2.5 g/L
• 最終pH值: 7.1±0.2 (25 °C)
• 應用: microbiology

描述

應用

For the confirmation of Clostridium perfringens.

準備報告

Suspend 20,3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense 8 ml portions into tubes with Durham gas collecting tubes for gas detection. Sterilize in autoclave at 121 ºC for 15 minutes. Before using add to each tube 0,5 ml of a 12 g/L solution of sodium metabisulfite and 0,5 ml of a solution of 10 g/L of ferric ammonium citrate. Both solutions have to be freshly prepared and sterilized.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3