11371517001

Swa I

from Staphylococcus warneri

• 分類程式碼代碼: 12352204

屬性

• 形狀: solution
• 包裝: pkg of 200 U (10 U/μl)
• 製造商／商標名: Roche
• 參數: 25 °C optimum reaction temp.
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

特異性

Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.

應用

Swa I is very useful for digesting GC-rich bacterial genomes.

DNA分析

Number of cleavage sites on different DNAs

• λ: 0
• φX174: 0
• Ad2: 1
• M13mp7: 1
• pBR322: 0
• pBR328: 0
• SV40: 1

單位定義

One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.

分析報告

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

• A: 0-10%
• B: 10-25%
• H: 100%
• L: 0-10%
• M: 0-10%

Activity in PCR buffer: Not tested

其他說明

仅用于生命科学研究。不可用于诊断。

僅套裝組件

• Enzyme Solution
• SuRE/Cut Buffer H 10x concentrated

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash