ST1711

Anti-DFHR Mouse mAb (2B10)

liquid, clone 2B10, Calbiochem^®

• 同義詞: Anti-Dihydrofolate Reductase
• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2B10, monoclonal
• 形狀: liquid
• 不包含: preservative
• 物種活性: rat, human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG2a
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DHFR(1719)

描述

一般說明

Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein.

Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells.

This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR.

免疫原

A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein

應用

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)

警告

Toxicity: Regulatory Review (Z)

外觀

In PBS, pH 7.2.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Negative Control
293T cells

Positive Control
HeLa cells, PC-12 Cells

其他說明

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable