SCC414

S462 Human Peripheral Nerve Sheath Tumor Cell Line

Human

• 同義詞: S462 Human Peripheral Nerve Sheath Tumor Cell Line
• 分類程式碼代碼: 41106514
• NACRES: NA.28

屬性

• product name: S462 Human Peripheral Nerve Sheath Tumor Cell Line,
• 生物源: human
• 包裝: pkg of >1X10⁶ cells
• 製造商／商標名: Sigma-Aldrich
• 增長模式: N/A
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: ≤ − 140°C

描述

一般說明

Malignant peripheral nerve sheath tumors (MPNSTs) are soft tissue sarcomas that arise from peripheral nerves and result in high rates of local recurrence and hematogenous metastasis. Half of MPNSTs occur in patients with neurofibromatosis type 1 (NF1), a common autosomal dominant multisystem disorder caused by mutations of the NF1 tumor suppressor gene located on chromosome 17q. Loss of function of the intact NF1 allele, due to loss of heterozygosity (LOH) and somatic mutations of the gene, is associated with dermal and plexiform neurofibroma formation. Approximately 10% of NF1 patients develop MPNST, of whom only 21% survive for five years after diagnosis.

應用

Each vial contains ≥ 1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Malignant peripheral nerve sheath tumors (MPNSTs) are soft tissue sarcomas that arise from peripheral nerves and result in high rates of local recurrence and hematogenous metastasis. Half of MPNSTs occur in patients with neurofibromatosis type 1 (NF1), a common autosomal dominant multisystem disorder caused by mutations of the NF1 tumor suppressor gene located on chromosome 17q. Loss of function of the intact NF1 allele, due to loss of heterozygosity (LOH) and somatic mutations of the gene, is associated with dermal and plexiform neurofibroma formation. Approximately 10% of NF1 patients develop MPNST, of whom only 21% survive for five years after diagnosis. Source S462 is an established human MPNST cell line from a clinically and genetically well-characterized NF1 patient, with a nonsense germline mutation in exon 37 of the NF1 gene, LOH of the NF1 gene, LOH of the p53 gene, and a homozygous missense mutation in exon 4 of the p53gene.As a verified MPNST cell line, S462 is a valuable tool for the further investigation of the biology and pathogenesis of this malignancy as well as for in vitro pharmacologic studies essential for the development of new therapies.

特點和優勢

S462 Human Peripheral Nerve Sheath Tumor Cell Line is a varified MPNST cell line and a valuable tool for the further investigation of the biology and pathogenesis of this malignancy.

儲存和穩定性

Cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

其他說明

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable