SCC292

HEK293 FUS Knockout Human Cell Line

Human

• 分類程式碼代碼: 41106514
• NACRES: NA.81

屬性

• product name: HEK293 FUS Knockout Human Cell Line,
• 生物源: human
• 包裝: vial of >1X10⁶ cells
• 製造商／商標名: Millipore
• 增長模式: N/A
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

Amyotrophic lateral sclerosis (ALS, Charcot or Lou Gehrig s disease) is a progressive neurodegenerative disease resulting in loss of motor control. ALS usually leads to mortality within 5 years of symptom onset.1 A subset of ALS cases are familial and lin

應用

• Each vial contains >1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Amyotrophic lateral sclerosis (ALS, also known as Charcot or Lou Gehrig′s disease) is a progressive neurodegenerative disease resulting in loss of motor control. ALS usually leads to mortality within 5 years of symptom onset.1 A subset of ALS cases are familial and linked to mutations in a handful of genes, including FUS (fused in sarcoma). FUS is a ribonucleotide binding protein that plays a role in RNA metabolism and is implicated in DNA repair pathways.2 Probing the effects of FUS point mutations is contributing to our understanding of the cellular mechanisms of ALS and the connections between genetic repair and neurodegeneration.

Source
HEK293 FUS KO cell line was derived from CRISPR-edited HEK293 cells.2 The parental HEK293 cell line was derived from cells from an aborted female fetus transfected with sheared adenovirus DNA.3

References
1. Zarei S, Carr K, Reiley L, Diaz K, Guerra O et al. Surg Neurol Int 2015; 6:171.
2. Wang H, Guo W, Mitra J, Hegde PM, Vandoorne T et al. Nat Commun 2018; 9:3683.
3. Graham FL, Smiley J, Russell, WC, Nairn R. J Gen Virol 1977; 36(1): 59-74.

特點和優勢

The HEK293 FUS knockout cell line was derived via CRISPR/Cas9-editing of HEK293 cells. Experimental validation of this line confirmed deletion of FUS with no off-target effects on expression of related proteins.2 This FUS knockout cell line permits direct study of the effects of FUS mutations via transfection of FUS variants. The HEK293 FUS knockout cell line represents a valuable system for the investigation of familial ALS in a robust cellular model.

儲存和穩定性

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

其他說明

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable