MABT1549

Anti-MT5-MMP/MMP24 Antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3D2 also termed M24N 3D2, monoclonal
• 分子量: calculated mol wt 70.46 kDa
• 物種活性: mouse, human
• 包裝: antibody small pack of 100 μg
• 技術: western blot: suitable
• 同型: IgG2aκ
• UniProt登錄號: Q9R0S2
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Mmp24(17391)

描述

一般說明

Matrix metalloproteinase-24 (UniProt: Q9R0S2; also known as MMP-24, Matrix metalloproteinase-21, MMP-21, Membrane-type matrix metalloproteinase 5, MT-MMP 5, MTMMP5, Membrane-type-5 matrix metalloproteinase, MT5-MMP, MT5MMP) is encoded by the Mmp24 (also known as Mmp21, Mt5mmp) gene (Gene ID: 17391) in murine species. MMP-24 is a single-pass type I membrane protein that is mainly expressed in neuronal cells of both central and peripheral nervous systems. It mediates the cleavage of N-cadherin and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. It is also involved in cell-cell interactions between nociceptive neurites and mast cells. MMP-24 is synthesized with a signal peptide (aa 1-41) and a propeptide (aa 42-128) that are subsequently cleaved off to generate functional MMP-24 (aa 129-618) or processed MMP-24 (aa 129-554). MMP-24 contains a cysteine switch motif (aa 110-117) that binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release results in the activation of enzyme.

特異性

Clone 3D2 is a rat monoclonal antibody that detects MT5-MMP/MMP-24. It targets an epitope within 16 amino acids from the extracellular domain.

免疫原

Ovalbumin-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human MT5-MMP.

應用

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.

Tested Applications

Western Blotting Analysis: A representative lot detected MT5-MMP/MMP24 in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

Western Blotting Analysis: A 1:200 dilution from a representative lot detected MT5-MMP/MMP24 in lysate from HEK293 cells over expressing MT5-MMP, but not in HEK293 cells over expressing MT1-MMP and in mock control HEK293 cell lysates (Negative). (Courtesy of Michael Willem, Biomedical Center, Ludwig Maximilian University of Munich, Germany).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MT5-MMP/MMP24, clone 3D2, Cat. No. MABT1549, is a rat monoclonal antibody that detects MT5-MMP and is tested for use in Western Blotting.

外觀

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at +2°C to +8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable