MABT1349

Anti-Desmoglein-2 Antibody, clone CSTEM28

• 同義詞: Cadherin family member 5;HDGC
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: CSTEM28, monoclonal
• 分子量: calculated mol wt 122.29 kDa; observed mol wt ~78 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; flow cytometry: suitable; western blot: suitable
• 同型: IgG2bκ
• 表位序列: Extracellular domain
• Protein ID登錄號: NP_001934
• UniProt登錄號: Q14126
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DSG2(1829)

描述

一般說明

Desmoglein-2 (UniProt: Q14126; also known as Cadherin family member 5, HDGC) is encoded by the DSG2 (also known as CDHF5) gene (Gene ID: 1829) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-2 is a single-pass type I membrane glycoprotein that is found in all tissues and in carcinomas. It serves as a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 50-609), a transmembrane domain (aa 610-634), and a cytoplasmic domain (aa 635-1118). Mutations in DSG2 gene have been linked to familial type of arrhythmogenic right ventricular dysplasia that is characterized y infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias. Desmoglein-2 levels are reported to be consistently upregulated in most cancers where it participates in the maintenance of intracellular junctions that prevents host immune responses. (Ref.: O Brien, CM., et al. (2017). Stem Cells. 35(3); 626-640).

特異性

Clone CSTEM28 is a mouse monoclonal antibody that detects Desmoglein-2. It targets an epitope within the extracellular domain.

免疫原

His-tagged recombinant fragment corresponding to the extracellular domain of human Desmoglein-2.

應用

Quality Control Testing

Evaluated by Western Blotting with recombinant human Desmoglein-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human Desmoglein-2.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Desmoglein-2 in Flow Cytometry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected Desmoglein-2 in Immunocytochemistry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

ELISA Analysis: A representative lot detected Desmoglein-2 in ELISA (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable