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重要文件

MABS2297

Sigma-Aldrich

Anti-TPO Antibody, clone 47

同義詞:

MoAb47, Thyroid peroxidase

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

47, monoclonal

分子量

calculated mol wt 102.96 kDa
observed mol wt ~120 kDa

純化經由

using protein G

物種活性

human

包裝

antibody small pack of 100

技術

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

同型

IgG1κ

表位序列

Unknown

Protein ID登錄號

UniProt登錄號

儲存溫度

2-8°C

基因資訊

human ... TPO(7173)

特異性

Clone 47 is a mouse monoclonal antibody that specifically detects Thyroid peroxidase.

免疫原

Human thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.

應用

Quality Control Testing

Evaluated by Western Blotting in human thyroid tissue extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Thyroid peroxidase (TPO) in human thyroid tissue extract.

Tested Applications

ELISA Analysis: A representative lot detected Thyroid peroxidase (TPO) in ELISA applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Radioimmunoassay: A representative lot detected Thyroid peroxidase (TPO) in Radioimmunoassay applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Thyroid peroxidase (Uniprot: P07202; also known as TPO) is encoded by gene TPO (Gene ID: 7173) in human. It is a single-pass type I membrane protein and has an Extracellular domain (19-846 aa), a Transmembrane domain ( 847-871 aa) and a Cytoplasmic domain (872-933 aa). It is primarily found in the thyroid gland and functions in the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine (T3 and T4). TPO is an integral part of the complex process by which the thyroid gland regulates metabolism and other vital bodily functions. Defects in this gene can cause congenital hypothyroidism, congenital goitre and thyroid hormone organification defect IIA. Moreover, TPO is a common target of autoimmune attacks in various thyroid disorders, such as Hashimoto′s thyroiditis and Graves′ disease, which can lead to disruptions in hormone synthesis and subsequent health issues.
(Ref.: Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8. (PMID: 2474433).

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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