MABS2292

Anti-SMCHD1 Antibody, clone 2B8

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rat
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2B8 (#8), monoclonal
• 分子量: calculated mol wt 225.65 kDa; observed mol wt ~225 kDa
• 物種活性: mouse
• 物種活性（以同源性預測）: human
• 包裝: antibody small pack of 100 μL
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2aκ
• UniProt登錄號: Q6P5D8
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (UniProt: Q6P5D8; also known as SMC hinge domain-containing protein 1) is encoded by the Smchd1 (also known as Kiaa0650) gene (Gene ID: 74355) in murine species. SMCHD1 is a homodimeric, non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture. It is shown to be essential for X chromosome inactivation. It is recruited to inactivated chromosome X by Xist RNA and acts by mediating the merge of chromatin compartments. It is reported to localize to the inactive X via the XistHnrnpK-polycomb repressive complex 1 pathway. In addition to its role in X inactivation, it also plays a role in the expression of several autosomal gene clusters that are subject to monoallelic expression. It promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments. SMCHD1 also plays a role in DNA repair. It localizes to sites of DNA double-strand breaks in response to DNA damage to promote the repair of DNA double-strand breaks. It acts by promoting non-homologous end joining and inhibiting homologous recombination repair. Heterozygous mutations in Smchd1 gene have been linked to facioscapulohumeral muscular dystrophy 2, an inherited muscular weakness syndrome. (Ref.: Jansz, N., et al. (2018). Cell Rep. 25(7); 1912-1923; Mould, AW., et al. (2013). Epigenetics Chromatin 6(1); 19; Blewitt, ME., et al. (2008). Nat. Genet. 40(5); 663-669).

特異性

Clone 2B8 is a rat monoclonal antibody that detects SMC hinge domain-containing protein 1 (SMCHD1). It targets an epitope within the ATPase activity domain in the N-terminal half.

免疫原

Recombinant fragment corresponding to 592 amino acids from the ATPase activity domain from the N-terminal half of murine Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1).

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type Mouse neuronal stem cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) in lysate from wild-type Mouse neuronal stem cells, but not in Smchd1 null cells.

Tested Applications

Western Blotting Analysis: A representative lot detected SMCHD1 in Western Blotting applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Immunofluorescence Analysis: A representative lot detected SMCHD1 in Immunofluorescence applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SMCHD1 in Immunoprecipitation applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SMCHD1, clone 2B8, Cat. No. MABS2292, is a rat monoclonal antibody that detects Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) and is tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

外觀

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable