MABS2234

Anti-ICA 512/RESP18 Antibody, clone 1348-D29-1

• 同義詞: Receptor-type tyrosine-protein phosphatase-like N;R-PTP-N;Islet cell antigen 512;ICA 512;Islet cell autoantigen 3;PTP IA-2
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1348-D29-1, monoclonal
• 分子量: calculated mol wt 105.85 kDa; observed mol wt ~110-140 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: immunofluorescence: suitable; western blot: suitable
• 同型: IgG2bκ
• 表位序列: N-terminal half
• Protein ID登錄號: NP_001186692
• UniProt登錄號: Q16849
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTPRN(5798)

描述

一般說明

Receptor-type tyrosine-protein phosphatase-like N (UniProt: P16849; also known as R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2) is encoded by the PTPRN (also known as ICA3, ICA512) gene (Gene ID: 5798) in human. ICA 512, a tyrosine phosphatase like intrinsic membrane protein, is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature form that contains a lumenal domain (aa 35-575), a transmembrane domain (aa 576-600), and a cytoplasmic domain (aa 601-979). Its lumenal region contains the RESP18 homology domain that is localized in amino acids 35-131. The mature form can undergo cleavage to generate an N-terminal fragment (ICA 512-NTF), a transmembrane fragment (ICA 512-TMF), and a cleaved cytosolic fragment (ICA 512-CCF). ICA 512 expression is restricted to neuroendocrine cells and is detected in the pancreas, brain, and pituitary. It is involved in the biogenesis and turnover of insulin secretory granules (SGs) in pancreatic islet β-cells . It plays a role in insulin secretion in response to glucose. It is required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. It plays a role in insulin secretion in response to glucose stimuli. It is also required for normal accumulation of secretory vesicles in hippocampus and pituitary. ICA 512-TMF is reported to regulate dynamics and exocytosis of insulin SGs. ICA 512-CCF is shown to translocate to the nucleus and promotes the expression of insulin and other granule-related genes. It enhances pancreatic b-cell proliferation by converging with signaling by STAT5B and STAT3. ICA 512-NTF containing the RESP18HD domain encodes for an intrinsically disordered region that acts as a condensing factor for the reversible aggregation of insulin and other β-cell proteins in a pH and zinc-regulated manner. (Ref.: Toledo, PL., et al. (2019). J. Biol. Chem. 294(21); 8564-8576; Mziaut, H., et al. (2008). Proc. Natl. Acad. Sci. USA. 105(2); 674-679; Trajkovski, M., et al. (2008). J. Biol. Chem. 283(48); 33719-33729).

特異性

Clone 1348-D29-1 is a mouse monoclonal antibody that detects ICA 512/RESP18. It targets an epitope within the RESP18 homology domain within the N-terminal fragment.

免疫原

GST-tagged recombinant protein fragment corresponding to the mature N-terminal fragment (without the signal peptide) of human ICA 512.

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from EndoC βH1 cells.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ICA 512/RESP18 in EndoC βH1 cells, but not in cells subjected to RNAi knockdown.

Tested Applications

Immunofluorescence Analysis: A representative lot detected ICA 512/RESP18 in Immunofluorescence applications (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

Western Blotting Analysis: A representative lot detected ICA 512/RESP18 in Western Blotting application (Toledo, P.L., et al. (2019). J Biol Chem. 294(21):8564-8576).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

外觀

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable