MABS1883

Anti-SUFU Antibody, clone 15G3.1

clone 15G3.1, from mouse

• 同義詞: Suppressor of fused homolog, SUFUH
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 15G3.1, monoclonal
• 物種活性: mouse, human
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_057253.2
• UniProt登錄號: Q9UMX1
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SUFU(51684)

描述

一般說明

Suppressor of fused homolog (UniProt: Q9UMX1; also known as SUFUH) is encoded by the SUFU (also known as UNQ650/PRO1280) gene (Gene ID: 51684) gene in human. SUFUH acts as a negative regulator in the hedgehog signaling pathway that is ubiquitously distributed in adult tissues. Three isoforms of SUFUH have been described that are produced by alternative splicing. Isoform 1 is detected in fetal brain, lung, kidney and testis and isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney. SUFUH is shown to down-regulate Gli1-and Gli2-mediated transactivation of target genes. Binding of SUFUH sequesters Gli proteins in the cytoplasm. It is a part of a corepressor complex that acts on DNA-bound Gli1 and may link Gli1 to beta-transducin repeat containing E3 Ubiquitin Protein Ligase (BTRC) and facilitate its proteasomal degradation. SUFUH regulates the formation of either the repressor form (Gli3R) or the activator form (Gli3A) of the full length form of GLi3 (Gli3FL). When complexed with SUFUH, Gli3FL remains in the cytoplasm and is maintained in a neutral state. Mutations in SUFU gene are known to cause medulloblastoma.

特異性

Clone 15G3.1 detects Suppressor of fused homolog (SUFU) in human and murine cells. It targets an epitope within 177 amino acids from the C-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Suppressor of fused homolog (SUFU).

應用

Anti-SUFU, clone 15G3.1, Cat. No. MABS1883, is a mouse monoclonal antibdoy that detects Suppressor of fused homolog (SUFU) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SUFU in human cerebellum, human testis, human pancreas, and human skin tissues.

Research Category
Signaling

品質

Evaluated by Western Blotting in NIH-3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SUFU in NIH-3T3 cell lysate.

標靶描述

~54 kDa observed; 53.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash