MABS1287

Anti-EPO-R Antibody, clone VP-2E8

clone VP-2E8, from mouse

• 同義詞: Erythropoietin Receptor
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: VP-2E8, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable
• 同型: IgG1κ
• NCBI登錄號: NP_000112
• UniProt登錄號: P19235
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EPOR(2057)

描述

一般說明

Erythropoietin receptor (UniProt: P19235, also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type I membrane protein that serves as a receptor for erythropoietin. It mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPO-R dimerizes triggering the JAK2/STAT5 signaling cascade. EPO-R has an extracellular domain (aa 25-250), a transmembrane domain (aa 251-273), and a cytoplasmic domain (aa 274-508). It is synthesized with a signal peptide (aa 1-24) that is cleaved to generate mature EPO-R. Upon stimulation following Erythropoietin (EPO) binding, EPO-R is phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins, which mediate cell proliferation. Phosphorylation on Tyrosine 454 is shown to be essential for PTPN6 interaction, and on Tyrosine 426 for PTPN11 interaction. Tyrosine 426 is also required for SOCS3 binding, however, Tyr-454/Tyr-456 motif is reported to be the preferred binding site. Ubiquitination of EPO-R at Lysine 81 is required for receptor internalization, whereas ubiquitination at Lysine 453 promotes trafficking of activated receptors to the lysosomes for degradation. Defects in EPOR gene are linked to familial erythrocytosis which is characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, and hypersensitivity of erythroid progenitors to erythropoietin.

特異性

Clone VP-2E8 recognizes EPO-R in human cells. It targets an epitope within the extracellular domain.

免疫原

A recombinant fragment corresponding to the extracellular domain of human EPO-R.

Epitope: extracellular domain

應用

Anti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation.

Flow Cytometry Analysis: 2 µg/mL from a representative lot detected EPO-R in UT-7 (EPO dependent) cells.

Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Flow Cytometry Analysis: A representative lot detected EPO-R in Flow Cytometry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Research Category
Signaling

品質

Evaluated by Immunocytochemistry in starved UT-7 cells.

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected EPO-R in starved UT-7 cells.

標靶描述

55.06 kDa calculated.

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.