MABS1268

Anti-MCRS1 Antibody, clone DON193B/C9

clone DON193B/C9, from rat

• 同義詞: Microspherule protein 1, 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: DON193B/C9, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_006328
• UniProt登錄號: Q96EZ8
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MCRS1(10445)

描述

一般說明

Microspherule protein 1 (UniProt: Q96EZ8; also known as 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS1) is encoded by the MCRS1 (also known as INO80Q, MSP58) gene (Gene ID: 10445) in human. MCRS1 is a nucleolar protein that is detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. Its highest levels are reported in the early S phase and it becomes undetectable in the G2 phase. Four isoforms of MCRS1 have been reported that are produced by alternative splicing. MCRS1 contains a nucleolar localization motif (aa 113-123), a coiled-coil domain (aa 301-335), and a forkhead associated (FHA) domain (aa 363-419). MCRS1 exhibits transforming activity, which is suppressed by phosphatase and tensin homologue (PTEN). It can modulate the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 forms a complex with Mi-2 beta, and RET finger protein (RFP) and co-localize in the Nucleolus. This complex activates rRNA promoter activity. (Ref.: Shimono, K., et al. (2005). J. Biol. Chem. 280(47); 39436-39447).

特異性

Clone DON193B/C9 detects Microspherule protein 1 in human cells.

免疫原

A mixture of GST-tagged full length recombinant human Microspherule protein 1 and GST-tagged C- and N-terminal recombinant fragments of human Microspherule protein 1.

Epitope: unknown

應用

Anti-MCRS1, clone DON193B/C9, Cat. No. MABS1268, is a rat monoclonal antibody that detects Microspherule protein 1 and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected MCRS1 in human tonsil, human spleen, HeLa cell, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., CNIO, Madrid, Spain).

品質

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected MCRS1 in 10 µg of HCT116 cell lysate.

標靶描述

~52 kDa observed; 51.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash