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MABN790

Sigma-Aldrich

Anti-Shank3 Antibody, clone 16E2.3

clone 16E2.3, from mouse

同義詞:

SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2, Shank3

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

16E2.3, monoclonal

物種活性

mouse

物種活性(以同源性預測)

human (based on 100% sequence homology)

技術

western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... SHANK3(85358)

相關類別

一般說明

SH3 and multiple ankyrin repeat domains protein 3 (UniProt Q9BYB0; also known as Proline rich synapse associated protein 2, ProSAP2, Shank postsynaptic density protein, Shank3) is encoded by the SHANK3 (also known as DEL22q13.3, PROSAP2, PSAP2, SCZD15, SPANK-2) gene (Gene ID 85358) in human. Shank3/ProSAP2 belongs to the ProSAP/Shank family of scaffolding proteins, which are important dendritic spines postsynaptic density (PSD) complex components that play an important role in linking membrane glutamate receptors to intracellular signaling proteins and actin cytoskeleton. Shank proteins interact with adaptor proteins and assembles glutamate receptors at the postsynaptic membrane via multiple domains, including ankyrin repeat, proline-rich, SH3, PSD95/disc-large/zona occludens-1 (PDZ), and SAM domains. Shanks, together with the adaptor protein Homer and the endocytic vesicle-associated protein dynamin-3, are required for proper localization of the endocytic zone in close proximity to the PSD. Shank-3 induces formation of functional dendritic spines in aspiny cerebellar neurons, whereas Shank-3 knockdown results in reduced spine density and size in cultured hippocampal neurons. SHANK3 gene mutations are known causes of mental retardation and autism spectrum disorders.

特異性

Expected to react with both isoform 1 (A) and 2 (B).

免疫原

Epitope: Internal (C-terminal half)
GST-tagged recombinant protein corresponding to the C-terminal half of human Shank3.

應用

Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-Shank3 Antibody, clone 16E2.3 is validated for use in Western Blotting for the detection of Shank3.

品質

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Shank3 in 10 µg of mouse brain tissue lysate.

標靶描述

~185 kDa observed. Multiplet banding pattern as a result of isoforms and/or posttranslational modifications. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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