推薦產品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
4E2, monoclonal
物種活性
human, mouse
技術
immunofluorescence: suitable
western blot: suitable
同型
IgG1
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... RAB10(10890)
一般說明
Ras related protein Rab-10 (Rab10) is encoded by the gene named RAB10 and is member of the small GTPase family of Rab proteins. Rab proteins play critical roles in intracellular trafficking from the formation of vesicles to their fusion with membranes. Specifically Rab10 is involved in regulating RME-1 in endocytic recycling as well as endoplasmic reticulum structural dynamics and morphology, and Rab10 also appears to be critical to formation and correct placement of proteins in such critical cellular structures as the basement membranes of during organ morphogenesis and basement membrane remodeling during development. Rab10 localizes to internal membrane structures like the endoplasmic reticulum and Golgi apparatus but in certain cells it can be found associated with cilia and other cellular structures as well. EMD-Millipore’s Anti-Rab10 mouse monoclonal antibody has been tested in western blot against human HeLa and mouse NIH3T3 cell lysates and in fluorescent immunocytochemistry on LOVO cells in culture.
免疫原
Purified recombinant fragment of human Rab10 expressed in E. Coli.
應用
Detect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Rab10 in LOVO cells.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
品質
Evaluated by Western Blotting in HeLa and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
標靶描述
~25 kDa observed. Uncharacterized bands may appear in some lysate(s).
外觀
Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
HeLa and NIH/3T3 cell lysates
HeLa and NIH/3T3 cell lysates
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Natalie Connor-Robson et al.
Neurobiology of disease, 127, 512-526 (2019-04-08)
Mutations in LRRK2 are the most common cause of autosomal dominant Parkinson's disease, and the relevance of LRRK2 to the sporadic form of the disease is becoming ever more apparent. It is therefore essential that studies are conducted to improve
Daniel R Gulbranson et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(39), E8224-E8233 (2017-09-13)
Rab GTPases are switched from their GDP-bound inactive conformation to a GTP-bound active state by guanine nucleotide exchange factors (GEFs). The first putative GEFs isolated for Rabs are RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) and its yeast homolog DSS4
Stephen J Gross et al.
Angiogenesis, 24(4), 789-805 (2021-05-07)
Collagen type IV (Col IV) is a basement membrane protein associated with early blood vessel morphogenesis and is essential for blood vessel stability. Defects in vascular Col IV deposition are the basis of heritable disorders, such as small vessel disease
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