MABN673

Anti-ARNTL Antibody, clone 1C5

ascites fluid, clone 1C5, from mouse

• 同義詞: Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, Short name=bHLHe5, Member of PAS protein 3, PAS domain-containing protein 3, b
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 1C5, monoclonal
• 物種活性: human
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1
• UniProt登錄號: O00327
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ARNTL(406)

描述

一般說明

Aryl hydrocarbon receptor nuclear translocator-like protein 1 or alternatively, Basic-helix-loop-helix PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5 (bHLHe5), Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3, and encoded by the human gene ARNTL,BHLHE5, BMAL1, MOP3 or PASD3, is a protein belonging to the basic helix-loop-helix-PAS domain containing transcription factor family. ARNTL protein is important for the molecular mechanisms behind circadian rhythms as it interacts with a number of CLOCK (Circadian Locomotor Output Cycles Kaput) family members as well as CLOCK itself to promote transcription of a number of proteins of the circadian clock. ARNTL interacts with CRY and CRY2, PER, TIMELESS, and other clock related genes as well as non-CLOCK proteins such as HIF-1alpha (HIF1A) and SUMO3. ARNTL appears critical for proper function of rhythms, as only knockouts of ARNTL in mice generate mice that have upset biological clocks and a shorter lifespan. ARNTL is highly expressed in the adult brain as well as skeletal muscle and heart and ARNTL is a nuclear localized transcription activator. EMD-Millipore’s Anti-ARNTL mouse monoclonal antibody has been tested in western blot against recombinant transfected protein and in paraffin embedded immunohistochemistry on human spleen tissue sections.

免疫原

Purified recombinant fragment of human ARNTL expressed in E. Coli.

應用

Anti-ARNTL Antibody, clone 1C5 is a highly specific mouse monoclonal antibody, that targets ARNT-Like 1 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ARNTL in human spleen tissue.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

品質

Evaluated by Western Blotting in ARNTL-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ARNTL-hIgGFc transfected HEK293 cell lysate.

標靶描述

~100 kDa observed. Uncharacterized bands may appear in some lysate(s).

外觀

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
ARNTL-hIgGFc transfected HEK293 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable