MABF3180

Anti-CCL4/MIP1β Antibody, clone 4G11.10.5

• 分類程式碼代碼: 12352203
• NACRES: NA.74

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4G11.10.5, monoclonal
• 分子量: calculated mol wt 10.21 kDa; observed mol wt ~8.0 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG2aκ
• 表位序列: Unknown
• Protein ID登錄號: NP_002975.1
• UniProt登錄號: P13236
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... CCL4(6351)

描述

一般說明

C-C motif chemokine 4 (UniProt: P13236; also known as G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, LAG-1, MIP-1-beta (1-69),Macrophage inflammatory protein 1-beta, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, ACT-2) is encoded by the CCL (also known as LAG1, MIP1B, SCYA4) gene (Gene ID: 6351) in human. CCL4/MIP1b is a secretory protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature form (aa 24-292) that can be further proteolytically cleaved at the N-terminal to generate MIP-1b (aa 26-92). It is present either as a homodimer or a heterodimer of MPIa and MIP1b. Both MIP-1a and MIP1β participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. Although they exert similar effects on monocytes their effect on lymphocytes differs; with MIP1a selectively attracting CD8+ lymphocytes and MIP1β selectively attracting CD4+ lymphocytes. MIP1b serves as a monokine with inflammatory and chemokinetic properties. It is one of the major HIV-suppressive factors produced by CD8+ T-cells that inhibits different strains of HIV-1, HIV-2, and simian immunodeficiency virus. CCR5 serves as a specific receptor for MIP1b. The processed form MIP-1b also retains the ability to induce down-modulation of surface expression of the chemokine receptor CCR5 and inhibit the CCR5-mediated entry of HIV-1 in T-cells. It also serves as a ligand for CCR1 and CCR2 isoform B.

特異性

Clone 4G11.10.5 is a mouse monoclonal antibody that detects human C-C motif chemokine 4 (CCL4/MIP1b ).

免疫原

Full-length human recombinant C-C-motif chemokine 4 (CCL4) (without the signal peptide).

應用

Quality Control Testing

Evaluated by Western Blotting with human recombinant C-C motif chemokine 4 (CCL4).

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human CCL4/MIP1b .

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected CCL4/MIP1b a in human small intestine and human adrenal gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CCL4/MIP1β, clone 4G11.10.5, Cat. No. MABF3180, is a mouse monoclonal antibody that detects CCL4/ MIP1β and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable