MABF3175

Anti-IL-5 Antibody, clone 4A1.6.15

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4A1.6.15, monoclonal
• 分子量: calculated mol wt 15.24 kDa; observed mol wt ~226 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG2aκ
• 表位序列: Unknown
• Protein ID登錄號: NP_000870.1?
• UniProt登錄號: P05113
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... IL5(3567)

描述

一般說明

Interleukin-5 (UniProt: P05113; also known as IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF) is encoded by the IL5 gene (Gene ID: 3567) in human. IL-5 is a disulfide-linked homodimeric secreted protein that is synthesized and secreted mainly by the activated CD4+ helper T cells of the Th2 subset, mast cells, and eosinophils. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein (aa 20-134). It serves as a powerful proinflammatory cytokine that is responsible for maturation, proliferation, activation, and migration of eosinophils. It is also a key factor in the upregulation of myeloid progenitors in the bone marrow after airway allergen challenge, and for trafficking from the marrow to the airways in upper or lower airways inflammation. Its biological effects are mediated via selective interaction with IL-5 receptor (IL-5R), which is composed of an a subunit (IL-5Ra) and a non-specific βc heterodimer. As a homodimer it binds to IL-5Ra expressed on eosinophil surface and recruits the βc dimer and induces the assembly of the IL-5/IL-5Ra/βc ternary complex. In the absence of IL-5, IL-5Ra is complexed with the intracellular JAK2, whereas the βc subunit is associated with JAK1. (Ref.: Pelaia, C., et al. (2019). Front. Physiol. 10; 1514).

特異性

Clone 4A1.6.15 is a mouse monoclonal antibody that detects human Interleukin-5 (IL-5).

免疫原

Full-length recombinant human Interleukin-5 (without the signal peptide).

應用

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-5.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-5.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-5 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable