MABF3072

Anti-SARS-CoV RBD Antibody, clone m396

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: m396, monoclonal
• 分子量: calculated mol wt 139.13 kDa; observed mol wt ~34 kDa
• 純化經由: using Protein A
• 物種活性: SARS coronavirus
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; neutralization: suitable; western blot: suitable
• 同型: IgG1
• 表位序列: Extracellular domain
• Protein ID登錄號: NP_828851
• UniProt登錄號: P59594
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: vaccinia virus ... s> S(1489668)

描述

一般說明

Spike glycoprotein (UniProt: P59594; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 1489668) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. Clone m396 is shown to neutralize SARS-CoV-2 virus by disrupting ACE2/RBD binding. It is also effective in neutralizing both SARS-CoV-1 and SARS-CoV-2 pseudoparticles. (Ref.: Ju, B., et al. (2020). Nature. 584(7819); 115-119; Cao, Y., et al. (2020). Cell. 182(1); 73-84).

特異性

Clone m396 is a human recombinant monoclonal antibody that detects SARS-CoV spike protein. It targets an epitope within the receptor binding domain.

免疫原

From human IgG phage library panned against the receptor-binding domain (RBD) of the SARS-CoV spike protein.

應用

Quality Control Testing

Evaluated by Western Blotting with His-tagged recombinant receptor binding domain of SARS-CoV spike protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant receptor binding domain of SARS-CoV spike protein.

Tested Applications

Neutralizing: A representative lot neutralized SARS-CoV in Neutralizing applications (Zhu, Z., et al. (2007). Proc Natl Acad Sci USA.;104(29):12123-8).

ELISA Analysis: Various dilutions of this antibody detected His-tagged SARS-CoV Receptor Binding Domain (RBD) of SARS-CoV Spike protein.

ELISA Analysis: A representative lot detected SARS-CoV RBD in ELISA applications (Ju, B., et al. (2020). Nature.;584(7819):115-119).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV RBD, clone m396, Cat. No. MABF3072, is a human recombinant monoclonal antibody that detects Spike protein of SARS-CoV and is tested for use in ELISA, Neutralizing, and Western Blotting.

外觀

Purified human recombinant monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable