推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1D10, monoclonal
物種活性
mouse
技術
RIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
mouse ... Rbfox3(52897)
一般說明
RNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.
免疫原
GST-tagged recombinant protein corresponding to mouse Fox1.
應用
Anti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Fox1 in 10 µg of adult mouse frontal cortex tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293).
RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711).
Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293).
RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711).
Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
品質
Evaluated by Western Blotting in adult mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate.
標靶描述
~43 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Sebastien M Weyn-Vanhentenryck et al.
Nature communications, 9(1), 2189-2189 (2018-06-08)
Alternative splicing (AS) is one crucial step of gene expression that must be tightly regulated during neurodevelopment. However, the precise timing of developmental splicing switches and the underlying regulatory mechanisms are poorly understood. Here we systematically analyze the temporal regulation
Brie Wamsley et al.
Neuron, 100(4), 846-859 (2018-10-16)
Cortical interneurons display a remarkable diversity in their morphology, physiological properties, and connectivity. Elucidating the molecular determinants underlying this heterogeneity is essential for understanding interneuron development and function. We discovered that alternative splicing differentially regulates the integration of somatostatin- and
Francesco Tomassoni-Ardori et al.
eLife, 8 (2019-08-21)
Brain-derived neurotrophic factor (BDNF) is a potent modulator of brain synaptic plasticity. Signaling defects caused by dysregulation of its Ntrk2 (TrkB) kinase (TrkB.FL) and truncated receptors (TrkB.T1) have been linked to the pathophysiology of several neurological and neurodegenerative disorders. We
Ainara Elorza et al.
Brain : a journal of neurology, 144(7), 2009-2023 (2021-03-17)
Correction of mis-splicing events is a growing therapeutic approach for neurological diseases such as spinal muscular atrophy or neuronal ceroid lipofuscinosis 7, which are caused by splicing-affecting mutations. Mis-spliced effector genes that do not harbour mutations are also good candidate
Alberto Parras et al.
Nature, 560(7719), 441-446 (2018-08-17)
Common genetic contributions to autism spectrum disorder (ASD) reside in risk gene variants that individually have minimal effect sizes. As environmental factors that perturb neurodevelopment also underlie idiopathic ASD, it is crucial to identify altered regulators that can orchestrate multiple
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