MABE878

Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3

clone 8E3, from rat

• 同義詞: ATP-dependent RNA helicase A, RHA, DEAH box protein 9, mHEL-5, Nuclear DNA helicase II, NDH II
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 8E3, monoclonal
• 物種活性: mouse, human
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_031868
• UniProt登錄號: O70133
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DHX9(1660)

描述

一般說明

RNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3′ to 5′ direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9.

應用

Research Category
Epigenetics & Nuclear Function

This Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.

Western Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).

品質

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract.

標靶描述

~140 kDa observed

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable