MABE425

Anti-DOT1L Antibody, clone 1A11.2

clone 1A11.2, from mouse

• 同義詞: Histone-lysine N-methyltransferase, H3 lysine-79 specific, Disruptor of telomeric silencing-1 like, DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1A11.2, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_115871
• UniProt登錄號: Q8TEK3
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DOT1L(84444)

描述

一般說明

Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC 2.1.1.43; UniProt Q8TEK3; also known as Disruptor of telomeric silencing-1 like, DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4) is encoded by the DOT1L (also known as KIAA1814, KMT4) gene (Gene ID 84444) in human. Disruptor of telomeric silencing-1 like (DOT1L) catalyzes the mono-, di-, and tri-methylation of histone H3 on lysine-79 (H3K79). DOT1L-mediated H3K79 methylation is associated with active transcription. Accumulating evidences indicate a role of DOT1L and H3K79 in many biological processes, including DNA damage response, cell cycle, embryonic cell development, as well as the initiation and maintenance of mixed lineage leukemia (MLL)-rearranged leukemia. MLL fusion proteins such as AF9, AF10, and ENL recruit DOT1L to their target gene loci to help promote the transcription of genes that contribute to leukemic transformation of hematopoietic progenitor cells. DOT1L also has been identified as a coactivator of Wnt signaling in colorectal cancer, and is shown to induce neoplastic transformation of immortalized human breast epithelial cells and increase tumor initiation and growth of human breast cancer cells. In addtion, DOT1L is reported to regulate androgen receptor activity via direct methylation.

特異性

Clone 1A11.2 detects both spliced isoforms of human DOT1L reported by UniProt (Q8TEK3).

免疫原

Epitope: Internal (N-terminal half).

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human DOT1L.

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

This Anti-DOT1L Antibody, clone 1A11.2 is validated for use in Western Blotting for the detection of DOT1L.

品質

Evaluated by Western Blotting in Jurkat nuclear extract.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DOT1L isoforms in 10 µg of Jurkat nuclear extract.

標靶描述

~185/165 kDa observed. 164.9 kDa (isoform 1; Q8TEK3-2) and 184.9 kDa (isoform 2; Q8TEK3-1) calculated.

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable