MABE1988

Anti-Cyclin D2 Antibody, clone D2-34B1-3

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: D2-34B1-3, monoclonal
• 分子量: calculated mol wt 32.90 kDa; observed mol wt ~28 kDa
• 純化經由: using protein G
• 物種活性: mouse, human
• 包裝: antibody small pack of 100 μg
• 技術: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2a
• 表位序列: Unknown
• Protein ID登錄號: NP_033959
• UniProt登錄號: P30280
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... ccnd2> Ccnd2(12444)

描述

一般說明

G1/S-specific cyclin-D2 (UniProt: P30280; also known and Cyclin D2;CCND2) is encoded by the Ccnd2 (also known as Cyl-2) gene (Gene ID: 12444) in murine species. Cell cycle progression is regulated by the sequential events that include activation and subsequent inactivation of cyclin dependent kinases (Cdk) and cyclins. Cyclins impart substrate specificity to the CDK/cyclin complex. D-type cyclins (D1, D2, and D3) are known to have critical roles in cell cycle progression, differentiation, and malignant transformation. They are encoded by distinct genes but show significant amino-acid similarity. D-type cyclins are shown to associate with cyclin-dependent kinase 4 and 6 (CDK4, CDK6) and promote phosphorylation and subsequent inactivation of the retinoblastoma tumor suppressor gene product, RB and RB-related proteins. Phosphorylation of RB allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase.

特異性

Clone D2-34B1-3 is a rat monoclonal antibody that detects Cyclin D2.

免疫原

GST-tagged recombinant murine Cyclin D2.

應用

Quality Control Testing

Evaluated by Western Blotting in Mouse spleen tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cyclin D2 in Mouse spleen tissue lysates.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Cyclin D2 in Immunofluorescence applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Western Blotting Analysis: A representative lot detected Cyclin D2 in Western Blotting applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Cyclin D2 in Immunoprecipitation applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Immunohistochemistry Applications: A representative lot detected Cyclin D2 in Immunohistochemistry applications (Rowan, S., et al. (2008). Dev Biol.;321(1):111-22).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Cyclin D2, clone D2-34B1-3, Cat. No. MABE1988, is a rat monoclonal antibody that detects G1/S-specific cyclin-D2 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

外觀

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable