MABE1526

Anti-ZCCHC11 (TUT4) Antibody, clone 9F6.1

clone 9F6.1, from mouse

• 同義詞: Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 9F6.1, monoclonal
• 物種活性: human, mouse
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_056084
• UniProt登錄號: Q5TAX3
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZCCHC11(23318)

描述

一般說明

Terminal uridylyltransferase 4 (EC 2.7.7.52; UniProt Q5TAX3; also known as TUTase 4, Zinc finger CCHC domain-containing protein 11) is encoded by the ZCCHC11 (also known as KIAA0191, TUT4) gene (Gene ID 23318) in human. TUTase 4 is an uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. It is involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets and also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, and miR-143 and miR-200c. It is reported to catalyze the 3′ uridylation of miR-26A that targets IL6 transcript to abrogate the silencing of IL6 transcript and promote cytokine expression. It may also participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB.

特異性

Clone 9F6.1 targets an epitope in the N-terminal region of ZCCHC11 (TUT4).

免疫原

GST-tagged recombinant human ZCCHC11 (TUT4) N-terminal fragment.

應用

Anti-ZCCHC11 (TUT4), clone 9F6.1, Cat. No. MABE1526, is a highly specific mouse monoclonal antibody that targets ZCCHC11 (TUT4) and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 4 µg/mL from a representative lot detected ZCCHC11 (TUT4) in 10 µg of C2C12 mouse myoblast and Jurkat human T-cell lysates.

品質

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected ZCCHC11 (TUT4) in 10 µg of PC3 human prostate cancer cell lysate.

標靶描述

~185 kDa observed. 185.2/81.14 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified.

Format: Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1