MABE1130

Anti-Hsp70/Hsp72 Antibody, clone 2H9

clone 2H9, from mouse

• 同義詞: Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP70.1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 2H9, monoclonal
• 物種活性: bovine, human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_776975.1
• UniProt登錄號: Q27975
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HSPA1A(3303)

描述

一般說明

Heat shock 70 kDa protein 1A (UniProt Q27975; also known as Heat shock 70 kDa protein 1, HSP70.1) is encoded by the HSPA1A (also known as HSP70-1) gene (Gene ID 281825 & 282254) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and to prevent apoptosis under various stress conditions.

特異性

Clone 2H9 reacts with the heat schock-inducible HSP72 (HSPA1A), but not the constitutively expressed HSPA8/Hsc70 (HSC73) (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449). Reactivity toward HSPA1B and HSPA2 (UniProt Q27965 and P34933) has not been determined.

免疫原

Bovine skeletal muscle Hsp70/Hsp72.

應用

Anti-Hsp70/Hsp72 Antibody, clone 2H9 is an antibody against Hsp70/Hsp72 for use in Western Blotting.

Immunoprecipitation Analysis: Clone 2H9 ascites fluid immunoprecipitated Hsp70/Hsp72 from human U-937 myeloid leukemia cell lysates (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Western Blotting Analysis: A representative lot detected Hsp70/Hsp72 expression and siRNA-mediated Hsp70 down-regulation in HeLa cells (Rohde, M., et al. (2005). Genes Dev. 19(5):570-582).
Western Blotting Analysis: A representative lot detected the heat schock-inducible Hsp70/Hsp72, but not the constitutively expressed HSPA8/Hsc70 (HSC73) in heat shock protein preparations from bovine skeletal muscle and human myocardium tissue (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
Western Blotting Analysis: A representative lot detected heat shock-induced Hsp70 expression in four human leukemia cell lines (U937, Jurkat, Molt4, HL60), but not in murine myeloma NSO cells (Lasunskaia, E.B., et al. (1997). Apoptosis. 2(2):156-163).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chaperones

品質

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Hsp70/Hsp72 in 10 µg of MCF7 cell lysate.

標靶描述

~70 kDa observed.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable