MABD127

Anti-FBXO15 Antibody, clone 3B11.1

clone 3B11.1, from mouse

• 同義詞: F-box only protein 15
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3B11.1, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgMκ
• NCBI登錄號: NP_001136430
• UniProt登錄號: Q8NCQ5
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FBXO15(201456)

描述

一般說明

F-box only protein 15 (UniProt Q8NCQ5) is encoded by the FBXO15 (also known as FBX15) gene (Gene ID 201456) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, U-box-type, and PHD-finger-type E3 ligases. The RING finger-type E3s include seven cullin-based E3s, such as SKP1–CUL1–F-box-protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C). F-box proteins use their N-terminal F-box motif to bind Skp1 and other motif(s) in their C-terminal region for substrates recognition and recruitment. F-box proteins are divided into the FBXW subclass that contains WD40 repeat domains, the FBXL subclass that contains leucine-rich repeat domains, and the F-box only (FBXO) subclass that contains not yet fully characterized substrate recognition domains. SCF-Fbxo15 is reported to mediate the ubiquitination of cardiolipin synthase (CLS1) for proteasomal degradation in human lung epithelial cells. PTEN-induced putative kinase 1 (PINK1) is shown to phosphorylate CLS1 and cause its release from the inner mitochondrial membrane to the outer membrane, making it accessible for Fbxo15-targeted ubiquitination.

特異性

Clone 3B11.1 recoginzes an N-terminal epitope present in spliced isoform 1 (UniProt Q8NCQ5-1), but absent in spliced isoform 2 (UniProt Q8NCQ5-2).

免疫原

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding an N-terminal sequence of human FBXO15.

應用

Anti-FBXO15 Antibody, clone 3B11.1 is an antibody against FBXO15 for use in Western Blotting.

Research Category
Stem Cell Research

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

品質

Evaluated by Western Blotting in human H9 embryonic stem cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected FBXO15 in 10 µg of human H9 embryonic stem cell lysate.

標靶描述

~57 kDa observed. 57.26 kDa calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Format: Purified

Purified mouse monoclonal IgMκ antibody in buffer containing PBS with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable