生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
7B5, monoclonal
物種活性
human
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... GATA3(2625)
一般說明
GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.
免疫原
Purified recombinant fragment of human GATA3 expressed in E. Coli.
應用
Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
品質
Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.
Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.
Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.
標靶描述
~75 kDa observed
外觀
Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
GATA3-hIgGFc transfected HEK293 cell lysate
GATA3-hIgGFc transfected HEK293 cell lysate
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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