MAB3776

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon^®, from mouse

• 同義詞: Murine Double Minute 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: SMP14, monoclonal
• 物種活性: rat, human, mouse
• 製造商／商標名: Chemicon^®
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable
• 同型: IgG1
• NCBI登錄號: NM_002392.2; NM_006878.2; NM_006879.2; NM_006881.2; NM_006882.2
• UniProt登錄號: Q00987
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MDM2(4193) ; mouse ... Mdm2(17246) ; rat ... Mdm2(314856)

描述

一般說明

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

特異性

Recognizes human MDM2.

免疫原

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

應用

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)

Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.

Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

外觀

Format: Purified

Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

分析報告

Control
POSITIVE CONTROL: Breast carcinoma

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable