MAB353A4

Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

clone rat-401, from mouse, ALEXA FLUOR^™ 488

• 同義詞: rat401, NES, Nbla00170, FLJ21841
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: rat-401, monoclonal
• 物種活性: rat, mouse
• 應無反應活性: human
• 技術: immunocytochemistry: suitable
• 同型: IgG1
• NCBI登錄號: NP_037119
• UniProt登錄號: P21263
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

描述

一般說明

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

特異性

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

免疫原

Embryonic rat spinal cord

應用

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

品質

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

標靶描述

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

外觀

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

儲存和穩定性

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析報告

Control
Mouse cortical neural stem cells (SCR029)

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable