推薦產品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
7-6C1, monoclonal
物種活性
rat, human, bovine
應無反應活性
mouse
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... TIMP1(7076)
一般說明
Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.
特異性
MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).
免疫原
Bovine TIMP-1
應用
Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH.
Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
聯結
Replaces: 04-1133
外觀
Format: Purified
Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin.
儲存和穩定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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