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Merck
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MAB3136

Sigma-Aldrich

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5

clone 4F5, Chemicon®, from mouse

同義詞:

NHE3

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

4F5, monoclonal

物種活性

rat, rabbit

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

特異性

Na+/H+ exchanger, isoform NHE3.

免疫原

Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.

應用

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)

Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.

Immunocytochemistry

Immunoprecipitation (use non-ionic detergent)

Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

聯結

Replaces: MAB3134

外觀

Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

儲存和穩定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Xiaochun Li et al.
Cells, 12(11) (2023-06-10)
The current prevailing paradigm in the renin-angiotensin system dictates that most, if not all, biological, physiological, and pathological responses to its most potent peptide, angiotensin II (Ang II), are mediated by extracellular Ang II activating its cell surface receptors. Whether
Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.
Li, XC; Hopfer, U; Zhuo, JL
American Journal of Physiology: Renal Physiology null
Elevated BSC-1 and ROMK expression in Dahl salt-sensitive rat kidneys.
Hoagland, KM; Flasch, AK; Dahly-Vernon, AJ; dos Santos, EA; Knepper, MA; Roman, RJ
Hypertension null
Brandon A Kemp et al.
Journal of the American Heart Association, 8(9), e012016-e012016 (2019-05-02)
Background Previous studies demonstrated that angiotensin (Ang) III , not Ang II , is the predominant endogenous agonist for Ang type-2 receptor ( AT 2R)-induced natriuresis in normal rats, and that hypertensive 12-week-old spontaneously hypertensive rats ( SHR ) lack

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