MAB1905

Anti-Laminin α1/β 1 Antibody, clone AL-4

clone AL-4, Chemicon^®, from rat

• 同義詞: Laminin A Chain
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AL-4, monoclonal
• 物種活性: mouse
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_005559.2
• UniProt登錄號: P25391; P55268
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Lama1(16772)

描述

特異性

As original characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin α1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele et al (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kDa β1/γ1 band and the 400 kDa α1 band of mouse EHS laminin. However the affinity for the 400 kDa band was very low. AL-4 also demonstrated strong binding the Laminin β1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin β1 chain, however the clone also recognizes the laminin α1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins α1 and β1, with the β1 reactivity being more prominent.

免疫原

Laminin isolated from mouse EHS tumor.

應用

ELISA/RIA

Western blot

Immunoprecipitation

Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Laminin α1/β 1 Antibody, clone AL-4 is validated for use in ELISA, IP, WB, IH(P) for the detection of Laminin α1/β 1.

外觀

Format: Purified

Purified from ascites by Protein G affinity chromatography. Purified immunoglobulin in 0.02M PB, 0.25M NaCl, pH=7.6, 0.1% sodium azide

儲存和穩定性

Maintain between 2 and 8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable