MAB1476-I

Anti-Chymotrypsin (Hu Pancreas) Antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: CHYMO10-104.10, monoclonal
• 分子量: calculated mol wt 28 kDa
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• 同型: IgG1
• UniProt登錄號: P40313
• 運輸包裝: dry ice
• 儲存溫度: -10 to -25°C
• 目標翻譯後修改: unmodified

描述

一般說明

Chymotrypsin is a serine endopeptidase that is synthesized in the pancreatic acinar cells as chymotrypsinogen that is cleaved by trypsin to generate active chymotrypsin. Activation of chymotrypsinogen involves proteolytic cleavage at two sites. The resultant three chains are held together by five disulfide bonds. Chymotrypsin is reported to preferentially cleave peptide amide bonds where the carboxyl side of the amide bond (the P1 position) is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine). The aromatic ring in their sidechain that fits into a ′hydrophobic pocket′ (the S1 position) of the enzyme. Two predominant forms of chymotrypsin have been described and are designated as chymotrypsin A and B. They display high degree of homology but have significantly different proteolytic characteristics. The active site residues in chymotrypsin are far apart in the primary sequence but are brought together in a crevice formed between the two protein domains.

特異性

Clone CHYMO10-104.10 is a mouse monoclonal antibody that detects human Chymotrypsin.

免疫原

Purified human pancreatic chymotrypsin.

應用

Anti-Chymotrypsin (Hu Pancreas), clone CHYMO10-104.10, Cat. No. MAB1476-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in ELISA and Immunohistochemistry (Paraffin).

ELISA: (Dilution is assay dependent)

Immunohistochemistry: 1:1000.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

外觀

Purified mouse monoclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.

儲存和穩定性

Maintain at -20°C in convenient aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 L or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable