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重要文件

MAB1281

Sigma-Aldrich

抗核抗体,克隆235-1

clone 235-1, Chemicon®, from mouse

同義詞:

HuNu

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
235-1, monoclonal
application:
ICC
IHC
IP
物種活性:
human
技術:
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
citations:
362

生物源

mouse

品質等級

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

235-1, monoclonal

物種活性

human

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

輸入

sample type neural stem cell(s)

同型

IgG1

運輸包裝

dry ice

目標翻譯後修改

unmodified

一般說明

干细胞或胚胎细胞疗法等细胞替代技术对许多疾病具有巨大的应用潜力。 人与动物细胞或组织之间的跨物种移植需要在宿主环境中鉴定和定位供体材料。抗核抗体是有用的细胞学和组织学标志物,可用于鉴定异种移植模型中的人类细胞。

特異性

人核单克隆抗体与人细胞特异性反应。 在免疫组化中,它对小鼠、大鼠、鸡没有反应性。 尚未测试其他哺乳动物或动物物种的反应性。
染色所有人类细胞类型的细胞核,使细胞核呈弥漫性染色。 中期细胞的染色体染色呈阴性。还可以染色灵长类动物的细胞核(Sanchez-Pernaute, 2005)。抗体不与小鼠或大鼠的细胞核反应。

應用

抗核抗体,克隆235-1已用于:免疫组织化学(1:200);人细胞核免疫标记(HuNu) (1:300);颌下腺的组织学和免疫荧光分析

外觀

形式:纯化
杂交瘤上清液。溶于PBS中的液体。不含防腐剂。
纯化的蛋白 A

儲存和穩定性

自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。

分析報告

对照
所有人类细胞类型

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Jun Lei et al.
American journal of obstetrics and gynecology, 212(5), 639-639 (2015-01-04)
Using a mouse model of intrauterine inflammation, we have demonstrated that exposure to inflammation induces preterm birth and perinatal brain injury. Mesenchymal stem cells (MSCs) have been shown to exhibit immunomodulatory effects in many inflammatory conditions. We hypothesized that treatment
Yuelin Zhang et al.
Cell transplantation, 21(10), 2225-2239 (2012-07-11)
Although transplantation of adult bone marrow mesenchymal stem cells (BM-MSCs) holds promise in the treatment for pulmonary arterial hypertension (PAH), the poor survival and differentiation potential of adult BM-MSCs have limited their therapeutic efficiency. Here, we compared the therapeutic efficacy
Human stem cell delivery for treatment of large segmental bone defects.
Dupont, KM; Sharma, K; Stevens, HY; Boerckel, JD; Garcia, AJ; Guldberg, RE
Proceedings of the National Academy of Sciences of the USA null
E Josue Ruiz et al.
Nature communications, 5, 3175-3175 (2014-01-17)
The signals that regulate stem cell self-renewal and differentiation in the lung remain elusive. Lung stem cells undergo self-renewal or lineage commitment to replenish tissue, depending on cross-talk with their environment. This environment, also known as the niche, includes mesenchymal
Ji Hyun Kim et al.
Scientific reports, 6, 38754-38754 (2016-12-08)
Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however

文章

免疫螢光法使用抗體結合的螢光分子來進行蛋白質定位、修改確認及蛋白質複合物可視化。

抗體與特定抗原結合,產生專屬的抗體抗原複合物。了解這種結合的本質及其作為分子標籤的研究用途。

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

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Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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