跳轉至內容
Merck
全部照片(1)

文件

LIF2010

Sigma-Aldrich

小鼠白血病抑制因子

10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

同義詞:

LIF, Differentiation-stimulating Factor, D Factor, Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF

登入查看組織和合約定價
全部照片(1)

About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

生物源

mouse

品質等級

300

化驗

>95% (HPLC and SDS-PAGE)

形狀

liquid

比活性

≥1 x 10(E8) U/mg

製造商／商標名

Chemicon®

濃度

10 μg/mL

技術

cell culture | stem cell: suitable

雜質

<0.1 ng/mg Endotoxin (of LIF)

輸入

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI登錄號

NM_008501

UniProt登錄號

P09056

運輸包裝

wet ice

應用

推荐的QC方案

M1生物测定

1.使用体外半固体琼脂培养物进行M1生物测定，该培养物在1 mL体积的DME（含20%FCS的0.3%琼脂）中含有约100个细胞。

2.加入100 μL样品或mLIF（等渗盐水中5%FCS中的10（E4）单位/mL），一式两份两倍系列稀释至35 mm培养皿。

3.将100μ L 5%FCS的等渗盐水加入到两个对照载玻片上。

4.在37°C、含10%CO2的完全湿润空气中培养7天。

5.对显示分化的菌落数进行评分（注：50单位定义为导致50%菌落分化的活性量）。

有关其他信息，请访问www.esgro-lif.com

LIF由CHEMICON International，Inc.生产，受美国专利号5,187,077、5,427,925、5,443,825、5,750,654 和 6,261,548，欧洲专利号 0285 448 和相关的外国专利的保护，并无法再销售。

單位定義

比活度：50 个单位定义为在 1 mL 琼脂培养物中诱导 50％ M1 克隆分化所需的小鼠 LIF 量。

分析報告

无菌和微质体测试均为阴性。
比活度：小鼠 LIF 的活性取决于诱导小鼠 M1 髓样白血病细胞分化的能力。在该测定法中，小鼠LIF的最低可检测浓度为 0.5 ng/mL

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Ting Ting Li et al.
Journal of veterinary science, 21(1), e13-e13 (2020-01-16)
Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement)
Kay Jüngling et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2100-2102 (2003-09-23)
The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous
Hsiao-Wen Lin et al.
Journal of neuroinflammation, 6, 7-7 (2009-03-10)
Ciliary neurotrophic factor (CNTF) has been regarded as a potent trophic factor for motor neurons. However, recent studies have shown that CNTF exerts effects on glial cells as well as neurons. For instance, CNTF stimulates astrocytes to secrete FGF-2 and
Eunhye Kim et al.
PloS one, 11(7), e0160289-e0160289 (2016-07-30)
Pigs provide outstanding models of human genetic diseases due to their striking similarities with human anatomy, physiology and genetics. Although transgenic pigs have been produced using genetically modified somatic cells and nuclear transfer (SCNT), the cloning efficiency was extremely low.
Xinrong Peng et al.
PloS one, 9(3), e90707-e90707 (2014-03-07)
Mice embryonic stem (ES) cells have enabled the generation of mouse strains with defined mutation(s) in their genome for putative disease loci analysis. In the study of cataract, the complex genetic background of this disease and lack of long-term self-renewal
查看所有相關論文

文章

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

白血病抑制因子 (LIF) 蛋白質

白血病抑制因子 (Leukemia Inhibitory Factor, LIF) 是一種幹細胞生長因子，用於多能小鼠胚胎幹細胞 (ES cells) 的體外培養。

條款

小鼠胚胎幹細胞培養程序與方案

幹細胞冷凍保存、冷凍保存幹細胞解凍和培養基製備的規程。

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

相關內容

含 Stericup® 快速釋放過濾器的 PES 膜的幹細胞測試

本文顯示含有 Stericup Quick Release 過濾器的 PES 膜為胚胎幹細胞的多能擴展提供了另一種選擇。

Stem Cell Testing of PES Membrane Containing Stericup® Quick Release Filters

This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務