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重要文件

HTS089RTA

Sigma-Aldrich

Ready-to-Assay LPA1 Lysophospholipid Receptor Frozen Cells

Human

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32011203
NACRES:
NA.84

產品名稱

Ready-to-Assay LPA1 Lysophospholipid Receptor Frozen Cells, Human LPA1 / EDG2 GPCR frozen cells for Calcium Flux FLIPR Assays.

生物源

human

製造商/商標名

Ready-to-Assay

技術

calcium flux assay: suitable

NCBI登錄號

檢測方法

fluorometric

運輸包裝

dry ice

一般說明

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA1 is widely expressed, with particularly notable expression in the ventricular zone of the embryonic cerebral cortex. Mice lacking LPA1 have changes in brain 5-HT and amino acids, and craniofacial abnormalities (Harrison et al., 2003). Millipore’s cloned human LPA1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at LPA1.

細胞系描述

GPCR Cell Lines
Host cells: Chem-1

生化/生理作用

GPCR Class: A
Protein Target: LPA1 / EDG2
Target Sub-Family: Lysophospholipid

成分

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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