一般說明
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
KiSS1 (also known as GPR54 and AXOR12) is a Gq-coupled GPCR that binds to a series of peptides, termed kisspeptins or metastin, derived from the KiSS-1 gene (Kotani et al., 2001; Muir et al., 2001). The KiSS-1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS-1 and GPR54 in inhibition of metastasis in human cancer (Harms et al., 2003). Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus (Messager et al., 2005; Shahab et al., 2005). In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism (Colledge, 2004; Seminara et al., 2004). Millipore′s cloned human KiSS1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant KiSS1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at KiSS1.
KiSS1 (also known as GPR54 and AXOR12) is a Gq-coupled GPCR that binds to a series of peptides, termed kisspeptins or metastin, derived from the KiSS-1 gene (Kotani et al., 2001; Muir et al., 2001). The KiSS-1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS-1 and GPR54 in inhibition of metastasis in human cancer (Harms et al., 2003). Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus (Messager et al., 2005; Shahab et al., 2005). In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism (Colledge, 2004; Seminara et al., 2004). Millipore′s cloned human KiSS1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant KiSS1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at KiSS1.
細胞系描述
- GPCR Cell Lines
- Host cells: Chem-1
應用
Human KiSS1 / GPR54 GPCR frozen cells for Calcium Flux FLIPR Assays.
生化／生理作用
- GPCR Class: A
- Protein Target: KiSS1/ GPR54
- Target Sub-Family: KiSS1-derived peptide
成分
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
免責聲明
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
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