ABT1838

Anti-Claudin-16

from rabbit, purified by affinity chromatography

• 同義詞: Paracellin-1, PCLN-1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: canine, human
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable
• 同型: IgG
• NCBI登錄號: NP_006571
• UniProt登錄號: Q9Y5I7
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CLDN16(10686)

描述

一般說明

Claudin-16 (UniProt: Q9Y5I7; also known as Paracellin-1, PCLN-1) is encoded by the CDLN1 (also known as PCLN1) gene (Gene ID: 10686) in human. Claudin-16 is a multi-pass membrane protein that span the plasma membrane 4 times, with their N- and C-termini located in the cytosol. It is selectively expressed at tight junctions of renal epithelial cells of the thick ascending limb of loop of Henle s where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of divalent cations. Claudin-16 may form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Over 20 different mutation sin CLDN1 gene have been reported that are linked to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), a renal Mg2+ and Ca2+ wasting disorder that ultimately leads to progressive kidney failure. (Ref.: Muller D et al. (2006). J. Clin. Endocrinol. Metab. 91(8):3076-3079).

特異性

This rabbit polyclonal antibody detects Claudin-16 in human and canine cells. It targets an epitope within 15 amino acids from the N-terminal region.

免疫原

Conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Claudin-16.

Epitope: extracellular domain

應用

Immunofluorescence Analysis: A representative lot detected Claudin-16 in Immunofluorescence applications (Kausalya, P.J., et. al. (2006). J Clin Invest. 116(4):878-91).

Inhibits Activity/Function: A representative lot blocked Clathrin-mediated endocytosis in MDCK cells (Muller, D., et. al. (2006). J Clin Endocrinol Metab. 91(8):3076-9).

Research Category
Cell Structure

This rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies.

品質

Evaluated by Immunocytochemistry in MDCK cells.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Claudin-16 in Madin-Darby Canine Kidney Epithelial (MDCK) cells.

標靶描述

33.84 kDa calculated.

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.54 M Tris, 0.2 M Glycine, 250 mM NaCl, pH 7.5 without azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash