推薦產品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, human
物種活性（以同源性預測）
porcine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
技術
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PHGDH(26227)
一般說明
3-PGDH (PHGDH) protein in an important enzyme in L-serine biosynthesis. 3-PGDH performs the first rate limiting step in the phosphorylated pathway of serine biosynthesis. 3-PGDH is also a biomarker in certain cancers that over express 3-PGDH. Moreover, 3-PGDH is the essential enzyme needed for cells to traverse the S phase and divide, and it plays a key role in T cell activation and proliferation for instance. Interestingly, even though 3-PGDH knockdown and reduced serine synthesis slows growth and proliferation of many cancer cells in vitro 3-PGDH knockdown does not affect tumor maintenance and growth in established breast cancer xenograft models, suggesting that 3-PGDH-dependent cancer cell growth may be context-dependent.
免疫原
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human 3-PGDH near the N-terminus.
應用
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected 3-PGDH in human cerebral cortex, human kidney, and human cerebellum tissue.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-3-PGDH Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 3-PGDH.
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected 3-PGDH in 10 µg of A431 cell lysate.
標靶描述
~54 kDa observed
外觀
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Weiyong Shen et al.
Glia, 69(8), 1966-1986 (2021-04-10)
The importance of Müller glia for retinal homeostasis suggests that they may have vulnerabilities that lead to retinal disease. Here, we studied the effect of selectively knocking down key metabolic genes in Müller glia on photoreceptor health. Immunostaining indicated that
Ting Zhang et al.
eLife, 8 (2019-05-01)
The human macula is more susceptible than the peripheral retina to developing blinding conditions such as age-related macular degeneration, diabetic retinopathy. A key difference between them may be the nature of their Müller cells. We found primary cultured Müller cells
Yanling Xin et al.
eLife, 11 (2022-12-03)
N 6-methyladenosine (m6A) is the most prevalent mRNA internal modification and has been shown to regulate the development, physiology, and pathology of various tissues. However, the functions of the m6A epitranscriptome in the visual system remain unclear. In this study
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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