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ABS194

Sigma-Aldrich

磷酸化PDHE1-A I型（Ser300）抗体

from rabbit, purified by affinity chromatography

同義詞:

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

100

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

物種活性（以同源性預測）

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

技術

immunocytochemistry: suitable
western blot: suitable

NCBI登錄號

NP_000275

UniProt登錄號

P08559

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pSer300)

基因資訊

human ... PDHA1(5160)

一般說明

在许多生物体中，丙酮酸脱氢酶复合体除了作为心脏底物选择的关键调节因子外，还在有氧能量生成途径中催化丙酮酸到乙酰辅酶a和二氧化碳的总体不可逆转化。它含有三种酶成分的多个副本：丙酮酸脱氢酶（E1）、二氢脂酰胺乙酰转移酶（E2）和脂酰胺脱氢酶（E3）。PDH通过丙酮酸脱氢酶激酶（PDK）介导的磷酸化和反馈抑制调节。

特異性

该抗体可识别在Ser300磷酸化的PDHE1-A 1型。

免疫原

对应于在Ser300磷酸化的1型人PDHE1-A的KLH偶联线性肽。
表位：磷酸化Ser300

應用

免疫细胞化学分析： 代表性批次的1:500稀释液在二氯乙酸未处理和已处理的HEK 293细胞中检测到PDHE1-A 1型。
该抗磷酸化PDHE1-A 1型（Ser300）抗体经验证可用于在WB、IC中检测磷酸化PDHE1-A 1型（Ser300）。

品質

已通过蛋白质印迹在二氯乙酸未处理和已处理的NIH/3T3细胞裂解液中进行了评估。

蛋白质印迹分析：0.5 µg/mL该抗体在10 µg二氯乙酸未处理和已处理的HEK 293细胞裂解液中检测到PDHE1-A 1型。

標靶描述

观测值〜43 kDa

分析報告

对照
二氯乙酸未处理和已处理的HEK 293细胞裂解液

其他說明

2024年CiteAb奖，授予在帕金森氏病研究方面取得成功的供应商

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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