ABS1655

Anti-PDI Antibody, arginylated (Nt-Asp18)

from rabbit, purified by affinity chromatography

• 同義詞: Protein disulfide-isomerase, Nt-Asp18 arginylated, Cellular thyroid hormone-binding protein, Nt-Asp18 arginylated, p55, Nt-Asp18 arginylated, P4Hbeta, Nt-Asp18 arginylated, PDI, Nt-Asp18 arginylated, Prolyl 4-hydroxylase subunit beta, Nt-Asp18 arginylate
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: chimpanzee (based on 100% sequence homology)
• 技術: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_000909
• UniProt登錄號: P07237
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... P4HB(5034)

描述

一般說明

Protein disulfide-isomerase (EC 5.3.4.1; UniProt P07237; also known as Cellular thyroid hormone-binding protein, p55, P4Hbeta, PDI, Prolyl 4-hydroxylase subunit beta) is encoded by the P4HB (also known as CLCRP1, ERBA2L, PDI, PDIA1, PO4DB) gene (Gene ID 5034) in human. Protein disulphide isomerase (PDI), BiP (GRP78 or heat shock 70 kDa protein 5), and calreticulin (CRT) are ER lumen folding factors that, together with other PTM enzymes, assist the process of newly synthesized proteins before their release from ER. PDI, BiP, and CRT themselves are subject to posttranslational signal peptide removal after their synthesis, exposing D18, E19, and E18 at the newly formed N-terminus, respectively. The N-terminus D and E residues of the mature proteins are permissive to arginine-tRNA-protein transferase 1/ATE1-catalyzed arginylation, forming arginylated mature proteins (R-DPI, R-BiP, and R-CRT) with altered cellular localization to allow their participation in non-ER functions. Although only the basal levels of R-PDI and R-CRT, but not R-BiP, are generally detectible in non-stimulated cells, upregulated N-terminal arginylation of all three proteins is observed upon cytosolic dsDNA exposure and proteasomal inhibition, indicating a shared role in innate immune responses to invading microbes. In addition, ER stress induction by thapsigargin treatment synergistically boosts proteasomal inhibition-induced upregulation of cellular levels of R-DPI, R-BiP, and R-CRT, suggesting that ER stress accelerates the supply of ER lumenal DPI, BiP, and CRT for N-terminal arginylation.

特異性

This rabbit polyclonal antibody specifically detected the immunogen peptide, but not the control peptide without arginylation at the N-terminal Asp18 (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).

免疫原

Epitope: N-terminus

Linear peptide corresponding to the N-terminal sequence of arginylated mature human PDI.

應用

Detect arginylated mature PDI using this rabbit polyclonal Anti-PDI, arginylated (Nt-Asp18), Cat. No. ABS1655, validated for use in ELISA, Immunocytochemistry, and Western Blotting.

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected PDI Nt-Asp18 arginylation induction in (18-hr 3 µM MG132 and 200 nM thapsigargin) treated HeLa cells (Courtesy of Yong Tae Kwon, Ph.D. , Seoul National University, Korea).

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected PDI Nt-Asp18 arginylation induction in (18-hr 3 µM MG132 and 200 nM thapsigargin) treated HeLa cells (Courtesy of Yong Tae Kwon, Ph.D. , Seoul National University, Korea).

ELISA Analysis: A representative lot detected the immunogen peptide, but not the control peptide without arginylation at the N-terminal Asp18 (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).

Western Blotting Analysis: A representative lot detected PDI Nt-Asp18 arginylation induction upon poly(dA:dT) transfection or arginine-tRNA-protein transferase 1 (ATE1) 1A7A isoform overexpression in HeLa cells. Combined proteasome inhibition and ER stress induction by an 18-hr 10 µM MG132 and 100 nM thapsigargin treatment synergized the two drugs′ efficacy toward cellular PDI Nt-Asp18 arginylation induction (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).

Research Category
Signaling

品質

Evaluated by Western Blotting in treated HEK293 cells.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDI Nt-Asp18 arginylation induction in 7.5 µg of lysate from (17-hr 3 µM MG132 and 200 nM thapsigargin) treated HEK293 cells.

標靶描述

~57 kDa observed. 55.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity purified.

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable