生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, rat
技術
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... GSTP1(2950)
一般說明
Glutathione S-transferase P (EC 2.5.1.18; UniProt P04906; also known as Chain 7, GST 7-7, GST class-pi) is encoded by the Gstp1 gene (Gene ID 24426) in rat species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTP exists as a homodimer composed of two Yf subunits.
特異性
Reacts with human and rat glutathione S-transferase P dimer (Yf/Yf) and monomer (Yf).
免疫原
Purified rat liver glutathione S-transferase P.
應用
Anti-Gstp1 Antibody is an antibody against Gstp1 for use in Western Blotting, Immunohistochemistry.
Research Category
Signaling
Signaling
Research Sub Category
Oxidative Stress
Oxidative Stress
Western Blotting Analysis: A representative lot detected glutathione S-transferase P in rat liver and human stomach tissue homogenates (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barrett′s oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barrett′s oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47).
Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et al. (2000). Cancer Res. 60(4):957–969).
品質
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate.
標靶描述
~25 kDa observed (Yf monomer).
外觀
Unpurified
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 產品選擇工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Faisal Bin Rashed et al.
Redox biology, 41, 101905-101905 (2021-03-01)
Tumour hypoxia negatively impacts therapy outcomes and continues to be a major unsolved clinical problem. Nitroimidazoles are hypoxia selective compounds that become entrapped in hypoxic cells by forming drug-protein adducts. They are widely used as hypoxia diagnostics and have also
Faisal Bin Rashed et al.
Redox biology, 52, 102300-102300 (2022-04-18)
Solid tumours are often poorly oxygenated, which confers resistance to standard treatment modalities. Targeting hypoxic tumours requires compounds, such as nitroimidazoles (NIs), equipped with the ability to reach and become activated within diffusion limited tumour niches. NIs become selectively entrapped
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務